Fig. 4
In vivo, high gametocyte conversion is associated with high ap2-g, msrp1 and gexp5 transcript levels. a, b The relative abundance (2−ΔΔCT) of the transcript levels of the indicated genes were determined for malaria patient samples in the high GCR (H, triangles, n = 20) and low GCR (L, circles, n = 20) cohorts using 18s rRNA (a) or sbp1 (b) RNA levels as the endogenous control. The reference sample was RNA from Pfgdv1.gfp.dd.T1 parasites grown in the absence of Shld1 to inhibit gametocyte production. c The relative abundance (2−ΔΔCT) of Pfs25 transcripts in the high GCR (H, triangles, n = 20) and low GCR (L, circles, n = 20) cohorts was determined using 18s rRNA or sbp1 RNA as the endogenous control and, again, the reference was RNA from Pfgdv1.gfp.dd.T1 parasites grown in the absence of Shld1 to inhibit gametocyte production. The experiment was performed in duplicate and the means and s.e.m. are shown. A Kruskal–Wallis test followed a Dunn’s multiple comparison test was used to compare between high and low converters and probability is indicated, p > 0.05 (ns), p < 0.05 (*) and p ≤ 0.0001 (****)
