Fig. 1

Inhibition of EZH2 activity preventively ameliorates DSS colitis. a Methods for DSS-induced acute colitis in C57BL/6 mice and GSK343 administration. b, c Body weight (b) and disease activity index (c) of mice that received regular drinking water alone (Water group, black dotted line) or 2.5% DSS-containing water (DSS group, blue dotted line), or 2.5% DSS combined with GSK343 injection (DSS + GSK343 group, red dotted line) (n = 15 per group). For statistical comparisons, asterisk indicates DSS vs. DSS + GSK343. d, e Colon length (d), representative hematoxylin and eosin (H&E) staining of distal colon sections, and corresponding histological scores (e) at day 11 after DSS induction (n = 15 per group). f Colonic inflammatory cytokine analysis from distal colonic tissue at day 7 after DSS induction (n = 5 per group). g Survival curves of mice treated with drinking water, 3% DSS, or 3% DSS plus GSK343 (n = 10 per group). Data are representative of three independent experiments. *P < 0.05 by log-rank test. b–f Data are representative of three independent experiments. Quantitative data are shown as the mean ± SEM. The statistical significance of differences was determined by two-way analysis of variance with Bonferroni post test (b, c) and one-way analysis of variance followed by Bonferroni post test (d–f). *P < 0.05, **P < 0.01, ***P < 0.001, n.s. = not significant. Source data are provided as a Source Data file