Table 1 Distribution of TRAV/TRAJ gene usage out of 16 cells sequenced from single-sorted MR1-5-OP-RU tetramer+ cells from WT splenic cultures and 40 cells from Traj33 KO splenic cultures
Traj33 genotype | TRAV | TRAJ | Representative CDR3α | Frequency |
|---|---|---|---|---|
WT | TRAV1 | TRAJ33 | CAVRDTNYQLIW | 16/16 |
KO | TRAV1 | TRAJ12 | CAVRDGGYKVVF | 22/40 |
KO | TRAV1 | TRAJ9 | CAVRDGGYKLTF | 9/40 |
KO | TRAV1 | TRAJ40 | CAVQTGNYKYVF | 9/40 |
