Fig. 2
From: An essential contractile ring protein controls cell division in Plasmodium falciparum
PfCINCH (P. falciparum coordinator of nascent cell detachment) is required for asexual stage replication. a Schematic of the TetR-DOZI system for translational repression. b Schematic of anhydrotetracycline (ATc) washout procedure and immunoblot of PfCINCH protein levels [+] and [−]ATc. Asterisk corresponds to the primary translation product of PfCINCHsmV5. c Replication curve of PfCINCHsmV5-Tet and parental parasites over three replication cycles [+] and [−]ATc. Mean ± SD for triplicate wells shown. d Staging curve of PfCINCHsmV5-Tet parasites [+]/[−]ATc over one replication cycle. Mean ± SD for triplicate wells shown. e Time-lapse differential interference contrast (DIC) micrographs of PfCINCHsmV5-Tet parasites [+]/[−]ATc at egress. Scale bars 5 µm. f Graph of normal and abnormal egress events observed from PfCINCHsmV5-Tet parasites [+]/[−]ATc. smV5, spaghetti monster V5; TetR, Tet-repressor. Source data are provided as a Source Data file
