Fig. 4

The Tg(iSuRe-Cre) allele increases the efficiency of inducible genetic modifications. a–c Representative confocal micrographs of P6 retina vessels labelled with IsolectinB4 (endothelial surface) and anti-ERG antibody (endothelial nuclei), obtained from animals with the genotype indicated to the left and induced with high-dose tamoxifen from P1 to P3. Images represent the observed phenotypic variability; EC number for each image is depicted in chart c (yellow dots). 20 vs 24 retina microscopic fields were analysed (n = 5 vs n = 6 animals per group). d Linear regression showing the high correlation (r²) between total Erg + EC number (phenotype) and the number of Erg + /MbTomato- cells. e, f Representative confocal micrographs of P6 retina vessels from animals with the genotype indicated to the left and induced with high-dose tamoxifen from P1 to P3. Images represent the phenotypic variability of animals with the same genotype. EC number and reporter expression frequency is indicated below the figures. A comparison of EC number and reporter expression frequency is depicted in chart for 24 vs 34 retina microscopic fields (n = 6 animals per group). Yellow dots in chart f represent the values for images in b and e.  g qRT-PCR analysis of Kdr mRNA levels from FACS-sorted liver ECs (n = 5 animals), or immunostaining analysis of liver sections of animals (n = 3) with the indicated genotype (see also Supplementary Fig. 2b). h Semi-quantitative competitive PCR showing the efficiency of Kdr, Rbpj and Nmyc deletion in FACS-sorted cells of adult mice with the indicated genotypes and induced with tamoxifen. Note that some cross-contamination of samples and DNA may occur during tissue dissociation and FACS of mutant and wild-type cells. i Semi-quantitative competitive PCR for the Notch1 floxed allele and a control genomic sequence showing the efficiency of the Ubc-CreERT2 induced Notch1 deletion in Tomato+ and Tomato- cells of the liver. j Semi-quantitative competitive PCR showing the efficiency of Rbpj gene inducible deletion in the Tomato− and Tomato+ cells of several distinct organs from Rosa26-CreERT2 mice induced with tamoxifen. k Epas1 mRNA relative levels (qRT-PCR) in LysM-Cre-reporter-expressing bone marrow-derived macrophages (n = 4). Scale Bars 200 μm. Error bars indicate StDev; **p < 0.001; ***p < 0.0001. Two-tailed unpaired t-test (4c) or ANOVA (4 g and 4k). Source data are provided as a Source Data file