Fig. 6
From: iSuRe-Cre is a genetic tool to reliably induce and report Cre-dependent genetic modifications
The Tg(iSuRe-Cre) allele is not leaky. a, b Charts showing the comparison of the frequency of cells with YFP or MbTomato-2A-Int-Cre expression when the Tg(iSuRe-Cre) or ROSA26LSL-YFP reporter lines are intercrossed with the indicated Cre lines (n = 5 animals or embryos per group). LysM-Cre and Vav1-Cre lines both recombine the ROSA26LSL-YFP reporter in non-desired cell types, but not the Tg(iSuRe-Cre) allele. c Confocal micrographs showing the recombination of the Tg(iSuRe-Cre) allele in Shh-GFP-Cre embryos. d, e The Tg(iSuRe-Cre) allele is not leaky and is induced only in the desired cell types/organs when the line is interbred with tissue-specific Cre-expressing lines (n = 3 animals per line). f Example readout illustrating the genes and regulatory regions surrounding the transgene integration site on chromosome 17. g Genomic DNA PCR detection of homozygous animals, found at the expected Mendelian ratio (n = 79). Error bars represent StDev. Scale bar 350 μm. Error bars indicate StDev. Source data are provided as a Source Data file
