Fig. 6
From: Functional rare and low frequency variants in BLK and BANK1 contribute to human lupus
BANKW40C impairs TRAF6 localization to p62 + sequestosomes. a Indirect immunofluorescence staining of HEK293T cells transfected with wild type BANK1-V5 and stained with a BANK1 antibody. Scale bar is 50 μm. DNA was stained with DAPI (blue). b Indirect immunofluorescence staining of HEK293T cells cotransfected with the cDNA constructs FLAG-TRAF6 and BANK1-V5 (top and middle panels) or with BANK1-V5 alone (bottom panel). Top panel: FLAG-TRAF6 was stained with a TRAF6 antibody (green) and BANK1-V5 was stained with a V5 antibody (red). Middle panel: FLAG-TRAF6 was stained with a TRAF6 antibody (red) and endogenous p62/SQSTM1 protein was labelled with a p62 antibody (green). Bottom panel: BANK1-V5 was stained with a BANK1 antibody (red) and endogenous p62/SQSTM1 protein was labelled with a p62 antibody (green). DNA was stained with DAPI (blue). Scale bar 50 μm. c Coimmunoprecipitation of BANK1-V5 and FLAG-TRAF6 from HEK293T cells demonstrating a two-way interaction. BANK1-V5 was pulled down using a BANK1 Ab and FLAG-TRAF6 with a FLAG antibody. TFRC transferrin receptor, isotype control, WCE whole-cytoplasmic extract
