Fig. 3
From: Atomic resolution cryo-EM structure of a native-like CENP-A nucleosome aided by an antibody fragment
Comparison of the cryo-EM and crystal structures of the CENP-A NCPs. a Side view (top) of the structures of cryo-EM NCPCENP-A, NAS (orange) and crystal NCPCENP-A, PAS (PDB ID: 3AN2) (blue) aligned using their core histones. Enlarged region (middle) shows the DNA end region. Residues Arg42 and K49 of CENP-A interact with the entry/exit DNA while residue Arg43 of CENP-A inserts into the DNA minor grove and Arg44 interacts with the backbone of the DNA close to the dyad region in the NCPCENP-A, NAS structure. The density map of the same region (bottom), contoured at 2.7σ. b Top view (top) of the cryo-EM NCPCENP-A, NAS (orange) and crystal NCPCENP-A, PAS (blue) DNA structures aligned using their core histones (not shown). Enlarged region shows the conformational difference of DNA in the two structures at the SHLs 1.7–2.5 region. c Top view (top) of the cryo-EM NCPCENP-A, NAS (orange) and crystal NCPCENP-A, PAS (blue) structures aligned using their core histones. Enlarged region (middle) shows the C-terminal tail (LEEGLG) region of CENP-A in the cryo-EM structure, which is missing in the X-ray structure. Density map of the same region (bottom), contoured at 3σ
