Fig. 4
From: Atomic resolution cryo-EM structure of a native-like CENP-A nucleosome aided by an antibody fragment
The CENP-A nucleosome has a more open DNA conformation. a The cryo-EM structure of NCPCENP-A, NAS (orange) and the crystal structure of human NCPH3.1, PAS (green, PDB ID: 5AV6) were aligned using the core histones. Enlarged region shows the comparison of the DNA end regions. b Sequence alignment of the human CENP-A and H3.1 αN region with secondary structure element (top). Close-up view of the αN helix regions of CENP-A (orange) and H3.1 (green) (bottom). Side-chains of charged residues in CENP-A or H3.1 that interact with nucleosome DNA ends are shown as sticks. Side-chain of CENP-A Trp47 corresponding to Ala47 in H3.1 packs against the side-chain of H4 Lys44
