Fig. 4
From: Structural basis for human sterol isomerase in cholesterol biosynthesis and multidrug recognition
The putative catalytic mechanism of EBP in the isomerization. a A top-scoring model of EBP and Δ8-sterol that was generated by Molecular Docking Simulations. The key resides of isomerization by EBP are shown as sticks. b Yeast complementation assay. EBP with the catalytic mutation cannot rescue the growth of ΔErg2 Saccharomyces cerevisiae. Growth of yeast expressing EBP mutant in the presence of 50 ng/ml concentrations of cycloheximide for 24 to 48 h. c Enzymatic activity assay in vitro. EBP catalyzes the conversion of deuterium labeled zymostenol (zymostenol-d7) to lathosterol-d7. LC-MS/MS was used for isolation and analysis zymostenol-d7 and lathosterol-d7. The SDS-PAGE shows the purified recombinant proteins used in the assays. Data shown are the mean ± SD of triple times determinations. Source data are provided as a Source Data file
