Fig. 3 | Nature Communications

Fig. 3

From: Pax3 cooperates with Ldb1 to direct local chromosome architecture during myogenic lineage specification

Fig. 3The alternative text for this image may have been generated using AI.

Identification of the PAX3 protein complex by mass spectrometry. a Scheme of tandem affinity purification of PAX3. b Western blot using anti-PAX3 antibody. c Silver staining of TAP samples from iPax3 and iPax3HaFlag 1-day induced EBs. d Unique peptides detected for proteins associated to chromatin looping and histone methylation. e Validation of PAX3 interaction with LDB1, ASH2L, and SMC1 in 1-day induced EBs. Images are representative of independent biological replicates (n = 3). f Immunofluorescence staining shows co-localization of PAX3 and LDB1 in the somites of E9.5 embryos. Upper panel: ×20 magnification. Lower panel ×63 magnification. PAX3 (green); LDB1 (white); Nuclei (blue). Scale bar: 50 µm. Source data are provided as a Source Data file

Back to article page