Fig. 7 | Nature Communications

Fig. 7

From: Pax3 cooperates with Ldb1 to direct local chromosome architecture during myogenic lineage specification

Fig. 7The alternative text for this image may have been generated using AI.

Ldb1 is required for hypaxial myogenesis. a Myogenic progenitor migration is impaired upon Ldb1 deletion in the Pax3 lineage. Representative immunostaining of cryosections from E10.5 Ldb1-deleted embryos reveals reduced numbers of PAX3+ cells (green) in the forelimb bud. MYF5 (white); nuclei (blue). Bar: 100 µm. Images are representative of at least three independent embryos (from distinct matings). b Quantification of PAX3 + signal from panel a. Graph represents mean + s.e.m. from three independent embryos (n = 3) (D). Student’s t-test *p < 0.05. c Immunostaining of E11.5 cryosections using MYHC antibody confirms the impaired migration observed in E10.5 embryos. MYHC (green); nuclei (blue). Bar: 100 µm. d Quantification of MYHC + signal from panel c. Graph represents mean + s.e.m. from independent embryos (n = 3). Student’s t-test ***p < 0.001. e Schematized model of Pax3-induced myogenic commitment. Pax3 regulates chromatin remodeling and looping by recruiting the histone methyltransferase (HMT) complex and Ldb1. Source data are provided as a Source Data file

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