Fig. 2
The Manβ1-4GlcNAc disaccharide originates from glycans produced by the OST. a A schematic diagram of two major biogenesis pathways of fOS in mammalian cells, OST hydrolysis of LLO and NGLY1 cleavage of N-glycans, and pharmacological inhibitors (red) that blocks either pathway. b FACE analysis of fOS pool isolated form Trex1−/− MEFs treated with 1 μM aclacinomycin (ACM) for 24 h. Quantification of the Manβ1-4GlcNAc disaccharide band is shown on the right (same below). c FACE analysis of fOS pool isolated form Trex1−/− MEFs treated with 30 μM Z-VAD or 50 μM Q-VD for 24 h. d, e Quantification of the Manβ1-4GlcNAc disaccharide in Trex1−/− MEFs treated with 100 μM of castanospermine (CSN, D) or 100 μM of kifunensine (KIF, E) for 24 h. Representative FACE gels are in Supplementary Fig. 4. Data are representative of at least three independent experiments. Error bars indicate SEM. Unpaired t-test
