Fig. 4
The Manβ1-4GlcNAc disaccharide activates an intracellular pathway. a A heat map showing immune gene expression profiles induced by Trex1−/− fOS pool or the Manβ1-4GlcNAc disaccharide. Permeabilized RAW264.7 cells were stimulated with 10 μM of the Trex1−/− fOS pool or the synthetic Manβ1-4GlcNAc disaccharide followed by quantitative RT-PCR analysis of each indicated mRNA. b, c Quantitative RT-PCR analysis of mCxcl10, mIfit1 and mIl10 mRNA in RAW264.7 cells (b) or BMDMs (c) that were either non-permeabilized or permeabilized by digitonin (as indicated on top), then cells were treated with mock or increasing amounts (1, 10 and 100 μM) of the Manβ1-4GlcNAc disaccharide for 24 h. d Multiplex ELISA analysis of cytokines and chemokines from experiments in b and c. Data are representative of at least two independent experiments. Error bars indicate SEM. Unpaired t-test
