Fig. 7
The Manβ1-4GlcNAc disaccharide enhances antibody response in vivo. a Multiplex ELISA analysis of cytokines and chemokines secreted by BMDCs treated with mock or increasing amounts (1, 10, and 100 μM) of the Manβ1-4GlcNAc disaccharide for 24 h. b A schematic diagram of Manβ1-4GlcNAc mouse immunization experiment. See Method for more details. c OVA-specific IgG1 antibody titre by ELISA using serum at day 21. Four vaccination groups are indicated on the bottom. MG, Manβ1-4GlcNAc. N = 5. d IFNγ ELISPOT assay using splenocytes at day 21. Five μg/ml of OT1 OVA (257-264), OT2 OVA (323-339), or SIY (SIYRYYGL) peptide (negative control) were used to re-stimulate the antigen specific T cells. IFN-γ production was determined 48 h later. Error bars indicate SEM. Unpaired t-test
