Fig. 1
From: Sialyl Lewisx-P-selectin cascade mediates tumor–mesothelial adhesion in ascitic fluid shear flow
Isolation and characterization of metastatic cancer stem cells. a Isolation of ovarian metastatic cancer stem cells. Blue circle, primary tumor. Red star, metastasis. b, c NOD/SCID mice engrafted with M-CSCs or NM-CSCs via b orthotopic or c i.p. injection. Left: representative bioluminescence image. Right: representative views of metastases in the peritoneal cavity. Arrows, metastases. Scale bar, 1 cm. n = 5 (orthotopic) or three mice (i.p) per group. d, e Representative image d of and H&E staining e of primary tumors from mice orthotopically transplanted with M-CSCs or NM-CSCs. Arrows, tumors. Scale bars = 1 cm d or 50 μm e. f Representative view of metastasis in mice engrafted with M-CSCs of patient samples. Arrows, metastases. Scale bar, 1 cm. n = 7 mice, samples collected from three patients. g Gene expression patterns of M-CSCs and NM-CSCS compared with metastatic (Met.) versus non-metastatic (Non-Met.) tumors from TCGA RNA-seq data of ovarian cancer patients. Left: heat map representing genes in M-CSC and NM-CSCs. Rows represent different genes and column represents each sample. Blue: downregulation; Red: upregulation. Right: Gene Set Enrichment Analysis of differentially upregulated genes in M-CSCs samples against the ranked gene list (from up- to downregulated) in TCGA metastatic ovarian tumors. X axis from left to right: ordered genes from up- to downregulation in TCGA metastatic tumors. Experiments were conducted two a–f or three g, n = 3 per group) times independently. Results are represented with mean ± SEM. Statistical analysis using unpaired Student’s t test. ns, not significant. **, P < 0.01
