Fig. 5
From: Rif1 S-acylation mediates DNA double-strand break repair at the inner nuclear membrane
Pfa4 associates with the inner nuclear membrane. a Pfa4 localizes to the endoplasmic reticulum (ER)43 but access to the inner nuclear membrane has not been demonstrated. b Cells with theta (θ) nuclei, induced by Nup53 overexpression-mediated inner nuclear membrane proliferation, were analyzed for inner nuclear membrane-association of Sec61-GFP, Hrd1-GFP, and Pfa4-GFP (arrowheads indicate GFP-positive θ structures). Representative Z-projections of confocal microscopy images are shown for cells treated or not with Zeocin. Scale bar: 5 μm. c Quantification of the analysis shown in panel b. Results from three independent experiments (n ≥ 400 total cells per strain and condition) are shown as mean values ± s.e.m.
