Fig. 3

Biochemical production using engineered Vibrio sp. dhg strains. a Ethanol production pathway. For ethanol production, pdc (pyruvate decarboxylase) and aldB (aldehyde dehydrogenase) from Z. mobilis were expressed under the P_VP15 promoter and a synthetic 5′-UTR. To improve yield, ldhA, frdABCD, and pflB were removed from the chromosome. b Fermentation profile of the VDHG411 strain during 24 h. Closed black circle, OD₆₀₀; closed inverted blue triangle, alginate; open blue triangle, mannitol; closed purple hexagon, acetate; green cross, lactate; closed red square, ethanol. c 2,3-BDO production pathway. The budABC operon from E. aerogenes was expressed under the P _VP15 promoter and a synthetic 5′-UTR. d Fermentation profile of the VDHG421 strain during 24 h. Closed black circle, OD₆₀₀; closed inverted blue triangle, alginate; open blue triangle, mannitol; closed purple hexagon, acetate; open orange diamond, acetoin; closed red square, 2,3-BDO. e Lycopene production pathway. For lycopene production, the crtEBI operon (encoding GGPP synthase, phytoene synthase, and phytoene desaturase) from L. purpurea was expressed under the P_VP13 promoter and a synthetic 5′-UTR. To improve the titer, dxs (DXS synthase), idi (IPP isomerase) and ispA (FPP syntase) from E. coli W3110 were additionally expressed. f Lycopene production by engineered Vibrio sp. dhg strains (VDHG131, VDHG132, and VDHG133) after 9 h. Abbreviations: DXP deoxyxylulose 5-phosphate, HMBPP 4-hydroxy-3-methylbut-2-enyl pyrophosphate, IPP isopentenyl pyrophosphate, DMAPP dimethylallyl pyrophosphate, FPP farnesyl diphosphate, GGPP geranylgeranyl pyrophosphate. Some reactions of glycolysis have been omitted for simplicity. Error bar indicates the standard deviations of three independent cultures (n = 3). White dot indicates actual data point