Fig. 6
Gut colonization capacities of Lm clones. Colonization capacity of clones was assessed by orally inoculating mEcad E16P KI female C57BL/6J mice62 by mixes of seven isolates of the same CC (tested CCs: CC1, CC2, CC4, CC6, CC9, and CC121), and by enumerating the total bacteria released in mice stools every day (see Methods section). a–c Each mouse was orally infected by 2 × 107 bacteria. n = a minimum of 4 mice per CC. a Number of bacteria of each CC released in mice stools per day. b, c Area under the curves of panel a per CC is shown in b and for hypervirulent (CC1, CC2, CC4 and CC6) and hypovirulent (CC9 and CC121) CCs in c, calculated for the interval between days 2 and 6. d–h Each mouse was orally infected by 2 × 108 bacteria. n = a minimum of three mice per CC. d Number of bacteria of each CC released in mice stools per day. e, f Area under the curves of panel d per CC is shown in e and for hypervirulent (CC1, CC2, CC4, and CC6) and hypovirulent (CC9 and CC121) CCs in f, calculated for the interval between days 2 and 4. g CFU counts for intestinal organs (small intestine, cecum, and colon) treated with gentamicin (100 μg/mL; 2 h) of mice killed on the 4th day after inoculation. h CFU counts for deeper organs (liver, spleen, mesenteric lymph nodes, and brain) of mice killed on the 4th day after inoculation. Colors are as in Fig. 1a. In boxplots, the boxes delimit the first (25%) and third (75%) quartiles, whereas the bars delimit the second quartile (median). Outliers are outside the whiskers. Mann–Whitney U test was used for statistical comparisons. ***p < 1.10−3; **p < 1.10−2; *p < 0.05. NS: Not significant. AUC: Area under the curve. Source data are provided as a Source Data file
