Fig. 10

AND-gated diamonds, but not AND-gated C1-FFLs, also evolve in negative controls. a Selection for filtering out a short spurious signal is the primary way to evolve AND-gated isolated C1-FFLs, but b AND-gated isolated diamonds also evolve in the absence of spurious signals. The selection conditions are the same as in Fig. 6, but we do not allow the signal to directly regulate the effector. In the “no spurious signal” and “harmless spurious signal” control conditions, motif frequencies are similar between low- and high-fitness genotypes (Supplementary Figs. 8 and 9), and so our analysis includes all evolutionary replicates. When scoring motifs, we exclude all two-mismatch TFBSs; results with inclusions, and for FFL-in-diamonds, are shown in Supplementary Fig. 10. Many non-AND-gated diamonds have the “no regulation” logic in Fig. 2, perhaps as an artifact created by the duplication and divergence of intermediate TFs; we excluded them from the “Any logic” and “Non-AND-gated” tallies in (b). See Supplementary Methods for the calculation of the y-axis. Data are shown as mean ± s.e.m. over evolutionary replicates. We reused data from Fig. 7 for “Spurious signal filter required (high fitness)”