Fig. 4

AND-gated C1-FFLs are associated with a successful response to selection. a Distribution of fitness outcomes across replicate simulations, calculated as the average fitness over the last 10,000 steps of the evolutionary simulation. We divide genotypes into a low-fitness group (blue) and a high-fitness group (red) using as a threshold an observed gap in the distribution. b High-fitness replicates are characterized by the presence of an AND-gated C1-FFL. “Any logic” counts the presence of any of the seven subtypes shown in Fig. 2b. Because one TRN can contain multiple C1-FFLs of different subtypes, each of which are scored, the sum of the occurrences of all seven subtypes will generally be more than “Any logic”. See Supplementary Methods for details on the calculation of the y axis. c The overrepresentation of AND-gated C1-FFLs becomes even more pronounced relative to alternative logic-gating when weak (two-mismatch) TFBSs are excluded while scoring motifs. Data are shown as mean ± s.e.m. of the occurrence over replicate evolution simulations