Fig. 5
Destroying the AND-logic of a C1-FFL removes its ability to filter out short spurious signals. a For each of the n = 25 replicates in the high-fitness group in Fig. 4, we perturbed the AND-logic in two ways, by adding one binding site of either the signal or the slow TF to the cis-regulatory sequence of the effector gene. b For each replicate, the fitness of the original motif (blue) or of the perturbed motif (red or orange) was averaged across the subset of evolutionary steps with an AND-gated C1-FFL and lacking other potentially confounding motifs (see Supplementary Fig. 4 and Supplementary Methods for details). Destroying the AND-logic slightly increases the ability to respond to the signal, but leads to a larger loss of fitness when short spurious signals are responded to. Fitness is shown as mean ± s.e.m. over replicate evolutionary simulations
