Fig. 9
Isolated C1-FFLs and diamonds rely on AND gates to filter out short spurious signals. We add a TFBS of either the fast TF or the slow TF to break the AND gate. This slightly increases the ability to respond to the signal, but leads to a larger loss of fitness when effector expression is undesirable. We perform the perturbation on a 8 of the 18 high-fitness replicates from Fig. 7b that evolved an AND-gated C1-FFL, b 4 of the 26 high-fitness replicates that evolved an AND-gated diamond in Fig. 7b, and c 15 of the 37 replicates that evolved an AND-gated diamond in response to selection for signal recognition in the absence of an external spurious signal (Fig. 10b). Replicate exclusion was based on the co-occurrence of other motifs with the potential to confound results (see Supplementary Methods for details). Fitness is shown as mean ± s.e.m. of over replicate evolutionary simulations, calculated as described for Fig. 5
