Fig. 1

Requirement for DNA in FANCD2 deubiquitination. a Schematic of ID2 ubiquitination and deubiquitination in our reconstituted system. b ID2 was first incubated with BL100 to ubiquitinate FANCD2, followed by treatment with USP1-UAF1 without or with benzonase. The error bars represent the mean+S.D. of data from three independent experiments. c Either benzonase or DNase I treatment abolished FANCD2 deubiquitination by USP1-UAF1. The error bars represent the mean+S.D. of data from three independent experiments