Fig. 4
From: An asymmetric allelic interaction drives allele transmission bias in interspecific rice hybrids
S1TPR-S1A4–S1A6 is a tripartite complex in the nucleus. a S1TPR (TPR, top), S1A4 (A4, middle), and S1A6 (A6, bottom) were fused with GFP and each was co-expressed with the nuclear localization signal marker (NLS-mCherry) in the rice protoplasts. The GFP fluorescence perfectly matched the mCherry fluorescence. Scale bars, 10 μm. b BiFC assays showed that, in the nuclei of rice protoplasts, A4 interacts with A6 and TPR (top and middle), but A6 does not interact with TPR (bottom). Scale bar, 10 μm. c, d Pull-down assays confirmed that A4 interacts with A6 (c) and TPR (d) in vitro. “*” indicates the MBP-A4 pull-down proteins (GST-A6 or GST-TPR) detected using the anti-GST antibody. M means 10–180 kDa protein size marker (Thermo Fisher Scientific, CA, USA). Source data of (c, d) are provided as a Source Data file
