Fig. 4

Effect of ABC transporter inhibition on γH2AX induced by doxorubicin. a, b Western blot analyses of γH2AX induced by increasing doses of doxorubicin (Dox). PaLung, PaKiT03, WI-38, HEK293T and MEF cells were pre-treated without or with 5 µM verapamil (Vera) (a) or 5 µM cyclosporin A (CSA) (b) for 30 min before the treatment with the increasing amount of DOX alone or together with Vera or CSA for additional 3 h. c Cells derived from different tissues of P. alecto were treated as in (a) and analysed by Western blotting. All results are representative of at least three experimental repeats. Tubulin was used as a loading control