Fig. 5
From: ABCB1 protects bat cells from DNA damage induced by genotoxic compounds
Identification of ABCB1 for the intrinsic doxorubicin efflux capability in bat cells. a Relative mRNA expression levels of ABCB1, ABCC1 and ABCG2 in human HEK293T and P. alecto kidney immortalised (PaKiT03) cell lines are shown by FPKM values. b qRT-PCR to determine the knockdown efficiency of each ABC transporter. PaKiT03 cells were transfected with control or two independent siRNAs of the indicated genes. mRNA expression of each gene is normalised to GAPDH mRNA expression, and presented relative to the control siRNA sample (mean ± SD from three experiments). c Doxorubicin (Dox) accumulation in PaKiT03 cells after siRNA knockdown of ABCB1, ABCC1 and ABCG2. Cells were treated with 10 µM doxorubicin for 3 h and analysed by flow cytometry. Verapamil (Vera) was used as a positive control for ABC transporter inhibition. Control represents the cells transfected with control siRNA. “Relative values” represents fluorescence intensities of the indicated condition relative to the fluorescent intensity of cells treated Dox alone (“−Vera”, the first bar) (mean ± SD from three experiments). Statistical significances were calculated using unpaired student’s two-sided t test. p < 0.05 is represented with * and p < 0.01 with **. d Western blot analysis of γH2AX in PaLung and WI-38 cells at different time points after a 3-h treatment with 10 µg/ml cisplatin (Cis). N.T stands for no treatment (starting at t = 0 h, indicated by arrow). The results are representative of three independent experiments. Tubulin was used as a loading control
