Fig. 1 | Nature Communications

Fig. 1

From: Mediator complex interaction partners organize the transcriptional network that defines neural stem cells

Fig. 1

Mediator complex interactome in neural stem cells. a Schematic representation of Mediator complex purifications from neural stem cells (NSCs) expressing Med15-FLAG. Mass spectrometry results of the three conditions were compared to select proteins that do not decrease in abundance upon treatments as chromatin-independent Mediator complex interactors. IP immunoprecipitation, MS Mass spectrometry. b Agarose gel with DNA from untreated NSC nuclear extract or nuclear extract treated with Benzonase or Ethidium Bromide, as indicated. DNA size markers (M) are indicated. Source data are provided as a Source Data file. c Interactome of the Mediator complex in NSCs. Novel Mediator interaction partners are in red, known Mediator interaction partners are in grey. Thickness of the edges gives an indication of the relative molar protein quantity observed in the purified Mediator complex samples

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