Fig. 2 | Nature Communications

Fig. 2

From: Mediator complex interaction partners organize the transcriptional network that defines neural stem cells

Fig. 2

Mediator complex interactor validation. a Immunoprecipitation (IP) of Carm1 and Med12 by a Carm1 antibody from NSC nuclear extract. Western blots are probed with the indicated antibodies. Control IP by rabbit IgG and 5% input are also shown. Source data are provided as a Source Data file. b Immunoprecipitation (IP) of Jmjd1c and Med12 by Jmjd1c antibody from NSC nuclear extract. Western blots are probed with the indicated antibodies. Control IP by rabbit IgG and 5% input are also shown. Source data are provided as a Source Data file. c Function in neural development of identified Mediator-interacting transcription factors in NSCs. References are provided in the Methods. d mRNA levels in NSCs of Mediator-interacting transcription factors (TFs) and the Top 600 highest expressed TFs in NSCs. The average emPAI scores, a semi-quantitative mass spectrometry-based measure of molar amounts, in the four Mediator complex purifications is shown for Mediator–interacting TFs

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