Fig. 1
From: Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d
Overall structure of the UrCas13d-crRNA binary complex. a Domain organization of UrCas13d. Catalytic residues of the R-X4-H motifs within two HEPN domains are labeled. b Schematic representation of the crRNA secondary structure. Bars between nucleotide pairs represent Watson-Crick base pairs and dot represents wobble G-U base pair. c Overall structure of the UrCas13d-crRNA binary complex shown in two different orientations, color-coded as defined in a and b. d Surface representations of the UrCas13d-crRNA binary complex shown the same views as in (c). e Structure of crRNA in the binary complex. Mg2+ is colored in wheat. f Electrostatic potential surface of the crRNA binding channel in the binary complex. Red, white and blue indicate negative, neutral and positive electrostatic potential surfaces, respectively. g Electrostatic potential surface of the NUC lobe in complex with crRNA. h Electrostatic potential surface of the REC lobe in complex with crRNA
