Fig. 5
From: Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d
Recognition of the crRNA spacer region by UrCas13d. a–d Details of the interactions between the crRNA spacer region and UrCas13d. Hydrogen bonds are shown as dashed lines. Domains and residues are colored according to Fig. 1. e, f Denaturing gels demonstrating the cleavage of target RNA by wild-type UrCas13d and the mutants in complex with crRNA
