Fig. 5
T-ALL-derived circulating serine and glycine can facilitate a cell survival benefit for leukemia supporting cells. a PHGDH, PSAT1, PSPH, and SHMT2 Affymetrix MAS 5.0 mRNA expression levels obtained from the R2 AMC genomics analysis and visualization platform (Meijerink dataset). Data were extracted and re-plotted comparing normal bone marrow (green, NBM, n = 7) control samples and pediatric T-ALL samples (orange, n = 117). b Metabolite levels measured by ion exchange chromatography in plasma samples from control mice (green) and mice xenografted with the indicated T-ALL samples (orange). Metabolites are reported in μmol/L. From left to right, the boxplots represent phospho-serine, serine and glycine. X# indicates the T-ALL PDX sample ID. Control mice n = 4 and PDX mice X11 n = 5, X12 n = 5, X13 n = 5, X14 n = 2, X15 n = 5. RPL10 R98S cases are indicated in blue. c Ion exchange chromatography determined serine and glycine levels in pooled conditioned media from either RPL10 WT or R98S Ba/F3 clones (n = 2 independent biological measurements of pooled CM n = 3). Data are represented as mean ± standard deviation. d 13C6-Glucose tracing of labeled serine and glycine released in the conditioned media of six Ba/F3 RPL10 WT clones versus five R98S clones. e Glycine and serine uptake rates comparing n = 6 Ba/F3 RPL10 WT clones versus n = 5 R98S clones. f Absolute viable cell counts of parental Ba/F3 cells to which conditioned medium (CM) taken from RPL10 WT or R98S cells was added, with or without addition of 20 μM serine. g Cell culture confluency plots illustrating the survival of bone marrow stromal cells and myeloid macrophages in presence and absence of 400 μM serine or glycine. Data are represented as mean ± standard deviation. All box-plots show the median and error bars define data distribution. Statistical analysis *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001. p-Values were calculated using a two-tailed Student’s t-test
