Fig. 2
Sfrp3 loss disrupts lumen formation and epithelial architecture of ducts. a Representative images of HE-stained mammary glands from Sfrp3+/+ and Sfrp3−/− virgin females at 8 weeks of age. Scale bar: 100 µm. b Quantitative analysis of the luminal filling phenotype showing an increase in the number of ducts with occluded lumen in Sfrp3−/− compared to Sfrp3+/+ females at 8 weeks of age. n = 2 Sfrp3+/+; 3 Sfrp3−/−. Data represent mean ± SD (*p < 0.05). c, d Immunofluorescence staining for Keratin 14 (K14) and E-cadherin (E-cadh) (c) and for Keratin8 (K8) and E-cadherin (d) on mammary sections of 8 weeks old females. The magnification of each bracketed area is shown on right side. Scale bar: 50 µm. e Immunofluorescence staining for E-cadherin and ZO1 on mammary sections of 8 weeks old females. c–e Images are representative of at least 10 MG sections analysed in 5 different experiments. Scale bar: 50 µm. See also Supplementary Fig. 2. Source data are provided as a Source Data file
