Fig. 5

Sfrp3 modulates Wnt signalling in the Drosophila wing imaginal disc. a Wingless (Wg) expression is altered in wing imaginal disc expressing Sfrp3-myc (grey) in the posterior compartment under Hh-Gal4 driver compared to the anterior compartment, which serves as control. Scale bar: 50 µm. b Fluorescence intensity profile of Wg (grey value; arbitrary units) along yellow lines in the anterior (ant) and posterior (post) compartment of the wing imaginal disc. c In Hh-Gal4>UAS-GFP, UAS-Sfrp3-myc imaginal discs, the Wingless target Senseless (Sens) expression is inhibited only in the posterior compartment (delimited by GFP expression) but not in the anterior compartment that serves as control. On the contrary, the Notch target Cut is normally expressed in these imaginal discs along the anterior-posterior axis. Scale bar: 50 µm. d Fluorescence intensity profile of Sens and Cut (grey value; arbitrary units) along yellow lines in the anterior (ant) and posterior (post) compartment of the wing imaginal disc. e Adult Nub-Gal4>UAS-Sfrp3-myc wings expressing Sfrp3 in the entire wing pouch and hinge show a lack of sensory organs along the margins that correspond to the dorsoventral boundary in imaginal discs. A high magnification of the bracketed area is shown. f In Hh-Gal4>UAS-GFP, UAS-Sfrp3-myc imaginal discs, the Wingless target Distalless (dll) expression is expanded in the posterior compartment (delimited by GFP/Sfrp3 expression) but not in the anterior compartment that serves as control. Scale bar: 50 µm. Fluorescence intensity profile of dll and GFP (grey value; arbitrary units) along the dorsal yellow lines of the wing imaginal disc. g In NRT-wg; Hh-Gal4>UAS-GFP, UAS-Sfrp3-myc imaginal discs, the Wingless target Senseless (Sens) expression is normal in the posterior compartment (delimited by GFP expression), similarly to the anterior compartment that serves as control. Scale bar: 50 µm. h Fluorescence intensity profile of NRT-wg and Sens a (grey value; arbitrary units) along yellow lines in the anterior (ant) and posterior (post) compartment of the wing imaginal disc. WT, wild-type. Scale bar: 200 µm. Images and fluorescent intensity profiles are representative of at least 5 images of wing imaginal discs analysed in 3 different experiments. For a, c, f, and g white dashed line delimitates posterior and anterior compartment