Fig. 1
Identification of a T cell-specific cis-regulatory element EBAB. a ChIP-seq visualization of H3K27ac in several mouse tissues. H3K27ac profiles from the thymus, spleen, bone marrow, liver, kidney, heart, brain, testis, and brown adipose tissues (BAT) are visualized using the UCSC genome browser (mm9). The EBAB region is highlighted. b DNase hypersensitivity sites (DHS) in the same locus shown in (a). DHS profiles from the thymus, T-Naïve CD4+, regulatory T (Treg) cells, spleen, and B cells (CD43− or CD19+) are visualized using the UCSC genome browser (mm9). The EBAB region is highlighted. c Schematic representation of ΔEBAB. Arrowheads indicate the primers listed in Supplementary Data 1. d Genomic PCR against the EBAB locus of WT, heterozygotes (EBAB+/−), and ΔEBAB mice. A representative gel-image of founder #44-derived DNAs is shown. See also Supplementary Fig. 2 for the results from #47- and #50-derived DNAs. e, f qPCR analysis for Bim (e) and Bub1 (f) on thymocytes, splenocytes, lung, liver, kidney, and pancreas. Data are pooled from five independent experiments (thymocyte, splenocyte; n = 5 WT and EBAB+/−–ΔEBAB littermate pairs, 7–17 weeks old, mean ± s.d.) or three independent experiments (lung, liver, kidney, pancreas; n = 3 WT & EBAB+/−–ΔEBAB littermate pairs, 10–17 weeks old). Each symbol represents an individual mouse; small horizontal lines indicate the mean. No statistically significant differences between WT and EBAB+/− and ΔEBAB were detected (P ≥ 0.05; unpaired two-tailed Student’s t test)
