Fig. 2
OsCd1 contributes to the Cd uptake and grain accumulation in rice. a Left: Schematic diagram of three CRISPR-oscd1 lines. Black rectangles represented exons of OsCd1, red rectangle represented the exon of target sequence. Right: Sequences of CRISPR-oscd1 alleles CR-1, CR-2, and CR-3. sgRNA targets sequences were showed and deletions are indicated by red dashes. b Growth of wild-type rice and three CRISPR-oscd1 lines after treating with and without 1 μM CdCl2 for 20 days. c, d, g, i, j Concentration of Cd in the shoot (c), root (d), straw (g), brown rice (i) and husk (j). e Growth of wild-type rice and three CRISPR-oscd1 lines after treated with and without 0.18 mg kg−1 Cd in pot-test. f Dry weight of straw wild-type rice and three CRISPR-oscd1 lines after treated with 0.18 mg kg−1 Cd in pot-test. h SR-μXRF images of Cd distribution in the longitudinal (upper) and latitudinal (lower) sections of rice grain. The emission intensity of each pixel was normalized using the beam intensity as reference. The CRISPR-oscd1 lines were shown in red and the wild-type line was shown in black. Error bars indicate standard deviation in the hydroponic experiment and standard error of mean in pot-test. Statistical comparison was performed by one-side t-test (*P < 0.05 and **P < 0.01). All data were compared with Nipponbare and designed with at least five replications and data points for all biological replicates are shown. Source data of Fig. 2c, d, f, g, i, and j are provided as a Source Data file
