Fig. 5
From: Genomic signatures of heterokaryosis in the oomycete pathogen Bremia lactucae

Sexual progeny from a cross between homokaryotic and heterokaryotic isolates form two half-sib groups. Kinship analysis based on SNPs segregating in sexual progeny generated by crossing SF5 (homokaryotic) with C82P24 (heterokaryotic). The first cluster delineates the majority of the offspring as one group of siblings derived from the same two parental nuclei (Subpopulation 1, SP 1). The second cluster delineates the remaining offspring as a second group of siblings derived from a different nucleus in C82P24 (Subpopulation 2, SP2). Relatedness of these two groups is consistent with having one parental nucleus in common derived from SF5. Relatedness of single-spore asexual derivatives of both isolates is also shown. Single-spore derivatives of C82P24 had a high relatedness to all other C82P24 derivatives and the original isolate. These derivatives and C82P24 were equidistant to all offspring, indicating the heterokaryotic C82P24 isolate had not been separated into homokaryotic components by generating single-spore derivatives. Source data is provided in the Source Data file