Fig. 6
Etomoxir treatment blocks AKR1B10-mediated metastasis. a 5 × 103 cells seeded into ultra-low adherence U-bottomed plates. Twenty-four hours post seeding 200 µM etomoxir or vehicle was added and tumour spheroid viability assessed on day 6. n = 8 ± SD. Equivalent results were obtained in two independent experiments. b BALB/c Nude mice injected intravenously with 1 × 106 cells and treated with vehicle or etomoxir (n = 5–7 per group). Lung tumour burden was assessed at the end of the experiment (day 55) by ex vivo IVIS imaging (dashed line indicates average radiance of aged-matched non-tumour bearing mice), lung weight and the mean % tumour area from three sections cut through the lungs 150 µm apart ± SEM. Representative ex vivo IVIS images and lung sections are shown. Scale bar, 1 mm. ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001; two-way ANOVA with Bonferroni post-testing (a, b)
