Table 1 CT26 neoantigens encoded by GAd-CT26-31 vaccine

From: Adenoviral vaccine targeting multiple neoantigens as strategy to eradicate large tumors combined with checkpoint blockade

ID

Gene

Neoantigen sequence

RNA reads

(mut/wt)

MAF (%)

MHC-I IC50

MHC-II score

1

Phf3

PGPQNFPPQNMF[G/E]FPPHLSPPLLPP

6/10

57

41.5

48.7

2

Zeb1

GAQEEPQVEPLD[L/F]SLPKQQGELLER

8/1

36

121.0

47.5

3

Trappc12

AVFAGSDDPFAT[A/P]LSMSEMDRRNDA

5/21

26

457.9

12.7

4

Aldh18a1

HSGQNHLKEMAI[P/S]VLEARACAAAGQ

12/7

51

13035.9

0.05

5

E2f8

ILPQAPSGPSYA[I/T]YLQPAQAQMLTP

3/5

32

320.5

6.8

6

Gid8

MSYAEK[P/S]DEITKDEWMEKL

7/31

26

67.0

49.8

7

Hdac2

GAGKGKYYAVNF[P/S]MRDGIDDESYGQ

29/30

40

39.48

40.5

8

Ttl

YRGADKLCRKAS[L/S]VKLVKTSPELSE

4/8

46

4768.9

0.2

9

Haus6

DSNLQARLTSYE[A/T]LKKSLSKIREES

29/9

33

18.2

7.49

10

Ndc1

HSFIHAAMGMAV[A/T]WCAAIMTKGQYS

3/1

70

15203.7

0.84

11

Glud1

LRTAAYVNAIEK[V/I]FKVYNEAGVTFT

353/89

68

141.15

9.4

12

G2e3

FEGSLAKNLSLN[S/F]QAVKENLYYEVG

12/15

53

467.9

21.1

13

Cad

DPRAAYFRQAEN[G/D]MYIRMALLATVL

11/3

52

2660.3

0.7

14

Dars2

LRSQMVMKMREY[L/F]CNLHGFVDIETP

6/7

46

3795.6

0.39

15

Smarcd1

DLLAFERKLDQT[I/V]MRKRLDIQEALK

16/2

46

11015.8

0.99

16

Zfp955b

IKREKCWKD[V/A]TY[S/P]ESFHTLESVPAT

2/16

45

271.8

9.2

17

Rwdd2b

GRSSQVYFTINV[S/N]LDLSEAAVVTFS

2/9

45

143.0

8.6

18

Slc20a1

KPLRRNNSYTSY[T/I]MAICGMPLDSFR

50/47

42

118.0

3.5

19

Ddx27

TTCLAVGGLDVK[S/F]QEAALRAAPDIL

13/16

41

8042.7

0.6

20

Top3a

IYEFDYHLYGQN[V/I]TMIMTSVSGHLL

1/1

41

124.9

11.4

21

Slc41a2

PDSFSIPYLTAL[G/D]DLLGTALLALSF

2/5

40

134.8

24.5

22

Ttc39a

YATILEMQAMMT[F/L]DPQDILLAGNMM

8/0

37

4108.0

0.2

23

Mtch1

SWIHCWKYLSVQ[G/S]SQLFRGSSLLFRR

160/226

36

13.46

2.2

24

Suv39h2

YDNKGITYLFDL[D/Y]YESDEFTVDAAR

2/7

36

344.48

33.6

25

Tomm70a

AQAAKNKGNKYF[K/Q]AGKYEQAIQCYT

17/41

35

459.4

5.2

26

Csnk2b

QPMLPIGLSDIP[G/D]EAMVKLYCPKCM

79/146

34

202.2

11.8

27

Caprin2

HRGAIYGSSWKY[S/F]TFSGYLLYQD

1/1

31

36.8

50.7

28

Dhx35

VIQTSKYYMRDV[T/I]AIESAWLLELAP

5/11

29

67.5

0.7

29

Xpot

PRGVDLYLRILM[A/P]IDSELVDRDVVH

17/53

28

41.5

4.7

30

Dclre1c

QIEQDALCPQDT[H/Y]CDLKSRAEVNGA

6/6

40

69.1

26.3

31

Noc3l

ALASAILSDPES[H/Y]IKKLKELRSMLM

5/3

60

101.2

10.7

  1. List of CT26 neoantigens encoded by GAd-CT26-31 vaccine. nAgs were selected according to (i) MHC class I (predicted IC50 ≤ 500 nm) and MHC class II (binding score ≤1) binding predictions, (ii) tumor allele frequency (MAF ≥ 25%), and (iii) RNA expression (≥1 mutated RNA read). The mutated amino acid is underlined. Predicted binders to MHC-I and II are highlighted in bold. MAF = minor allele frequency
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