Fig. 4

Q205 in Orc1 compensates for the loss of H4 tail interactions. a Interaction between Orc1 Q205 (purple) and H3 D77 (blue). b Effect of Q205D mutation on Orc1 BAH binding to unmodified nucleosome (WT nuc) as measured by EMSA. c Model for Orc1 BAH or Sir3 BAH interactions with nucleosome. Interaction strength is represented by dashes. d Binding of Sir3 D205Q to unmodified and H4K16ac nucleosomes measured by EMSA. e Binding of Sir3 D205Q with Orc1-swapped residues in the Lid of Loop 2 (Sir3 Lid D205Q) to unmodified and H4K16ac nucleosomes measured by EMSA shows that mutation of these residues reduces the discrimination for H4K16ac. Each data point and error bar represent the mean ± s.d. from three or more independent experiments. The standard errors of dissociation constants (K_D) are indicated