Fig. 2
From: Insights into real-time chemical processes in a calcium sensor protein-directed dynamic library
Aniline derivatives tested as DCL hydrazone exchange catalysts27. a DCL building blocks and library conditions at physiological pH and low temperature, b Aniline derivatives used as catalysts. c Time course formation of compound 3b using an aldehyde concentration of 0.09 μM in 20 mM Tris buffer (pH 7.4), acylhydrazide 2b (0.27 μM), T = 4 °C, 5% DMSO in the absence of the catalyst (red dots), and in the presence of 15 mM of aniline (green dots), p-anisidine (blue dots) and p-phenylendiamine (black dots). d Kinetic parameters (Kobs and t1/2) of acylhydrazone 3b formation calculated for a pseudo-second-order rate equation in the absence or in the presence of different catalysts (Supplementary Fig. 1 and Supplementary Methods). Mean ± SD from three independent experiments. The right column shows the rate enhancement of catalysts relative to the uncatalyzed samples. p-aminophenol was discarded as it got quickly transformed into the quinone derivative. Source data are provided as a Source Data file
