Fig. 3
From: Insights into real-time chemical processes in a calcium sensor protein-directed dynamic library
Acylhydrazone exchange reaction mechanism. a Formation of acylhydrazone 3b in the absence (i) and presence (ii) of p-anisidine. b Real-time 1D 1H NMR series recorded as a function of time (only a small subset of the resulting spectra hereby shown) of the reaction of 1 (50 mM) and 2b (50 mM) in Tris buffer D2O/DMSO-d6 (1:4) at 298 K (500 MHz). Colored arrows show the positions of specific signals from products and intermediates. The horizontal offset in ppm is 0.1. Note that there is a small fraction of the hydrated aldehyde in the spectra. c Plots of four selected UF-2D-TOCSY NMR spectra taken from the 500 experiments acquired (1:2b:p-anisidine at 1:1:0.5 in Tris buffer D2O/DMSO-d6 (1:4) at 298 K (500 MHz). Cross-peaks from the Schiff-base intermediate and the final step of formation of 3b are depicted in red and purple respectively
