Fig. 5

ALP-dependent cell membrane anchorage of DNA-lipid-P. a Schematic illustration of ALP-dependent cell membrane anchorage of DNA-lipid-P. b Mean fluorescence intensity of HepG2 cells treated with TAMRA-labeled DNA, DNA-lipid, DNA-lipid-P or DNA-lipid-P + Na₃VO₄ (2 mM) for one hour at 37 °C. Error bars indicate standard deviation. c Confocal fluorescence microscopy imaging of HepG2 cells treated with 1 μM TAMRA-labeled DNA, DNA-lipid, DNA-lipid-P or DNA-lipid-P + Na₃VO₄ (2 mM) for one hour at 37 °C. Scale bar is 30 μm. d Hybridization-induced fluorescence quenching of membrane-anchored TAMRA-labeled DNA-lipid or DNA-lipid-P with Dabcyl-cDNA