Fig. 4
The effect of SCNG-based RGD linkers unfolding on the adhesion and spreading of stem cells. a Synthesis and substrate conjugation of dynamic supramolecular ADA@CD-SCNG-RGD through a click reaction. b The folded ADA@CD-SCNGs on a glass substrate can be observed as particle-like structures (white dots) by SEM. The ADA@CD-SCNG-RGD linkers in the folded state provide a short RGD tether length (λ) and limited mobility (κ), which favour the formation of mature focal adhesions (FAs) and the spreading of cells on the substrate. c The unfolding of the SCNGs resulted in the disappearance of these white dots, and the unfolding of the ADA@CD-SCNG-RGD linkers led to an increased RGD tether length (λ) and mobility (κ), thereby hampering the formation of FAs and the spreading of cells. The scale bars are 100 nm
