Fig. 5
H3K27me3 and H3K9me3 mark the same transposable element copies in an Ezl1-dependent manner. a H3K9me3, H3K27me3, and H3K4me3 enrichment at individual copies from different TE families at an early stage of development (at 10 h after the onset of sexual events) in wild-type cells as measured by ChIP-qPCR. Quantitative data are expressed as the percentage of ChIP over Input. ACTIN, GAPDH, EE PPASE, ST PPASE, ST KINASE, and HELICASE are control genes (Supplementary Table 4 for accession numbers and primers). According to the RNA-seq analysis, Anchois (TIR), RT31010; RT42890; RT50144; RT48784–1; RT48639–1; RT48639–2 (LINEs) are significantly upregulated upon EZL1 RNAi, whereas RT25765; RT12275; RT32989; RT43773; RT48784–1 (LINEs) are not. Circles (black for genes; white for TEs) indicate the individual data points. Horizontal bars represent the mean of biological replicates. b H3K9me3, H3K27me3, and H3K4me3 enrichment at the same individual TE copies at a late stage of development (50 h after the onset of sexual events) upon PGM (top panel) or EZL1 (bottom panel) RNAi as measured by ChIP-qPCR. Data are represented as in a. c Heatmap of H3K27me3, H3K9me3, and H3K4me3 enrichment for 61 TE families determined by ChIP-seq of two biological replicates. Colors represent the fold-change (log2) in a given TE family for each histone mark relative to input. Source data are provided as a Source Data file
