Fig. 6
From: m6A mRNA demethylase FTO regulates melanoma tumorigenicity and response to anti-PD-1 blockade
FTO regulates its target gene expression through suppressing m6A/YTHDF2-mediated mRNA decay. a qPCR analysis of mRNA levels in Mel624 stable cells of shNC or shFTO. b–d qPCR analysis of the mRNA levels of PD-1 (PDCD1) (b), CXCR4 (c), and SOX10 (d) in Mel624 cells with or without FTO knockdown, in combination with siRNA knockdown of the m6A readers YTHDF1-3. e–g qPCR analysis of the mRNA stability of PD-1 (PDCD1) (e), CXCR4 (f), and SOX10 (g) in Mel624 cells with or without FTO knockdown. h-j qPCR analysis of the mRNA stability of PD-1 (PDCD1) (h), CXCR4 (i), and SOX10 (j) in Mel624 cells with or without FTO knockdown in combination with or without siRNA knockdown of YTHDF2. k, l Cell proliferation (k) and migration (l) analysis in Mel624 with or without knockdown or forced overexpression of YTHDF2. m Tumor growth of Mel624 cells with or without knockdown or forced overexpression of YTHDF2 after subcutaneous injection in nude mice (n = 3). Data are shown as mean ± S.E. (a–k), or mean ± S.D. (l and m) (n ≥ 3). *P < 0.05; **P < 0.01; Student’s t-test
