Fig. 2
From: Identification of a potent benzoxaborole drug candidate for treating cryptosporidiosis
AN7973 inhibits intracellular Cryptosporidium replication. a Structure of the 6-carboxamide benzoxaborole lead AN7973. b In vitro efficacy of AN7973 against various C. parvum isolates and the C. hominis TU502 isolate grown within HCT-8 cells. Nitazoxanide (NTZ) is included as a positive control. BGF Bunch Grass Farms, UAZ University of Arizona. The percent fetal bovine serum present in the medium and number of replicates for each assay are given in parentheses. Data are the mean and SD. Assays for c through d were performed in 10% FBS. c Effect of AN7973 on intracellular DNA synthesis. C. parvum (Bunch Grass Farms Iowa isolate) infected host cell monolayers were incubated in the presence of the thymidine analog EdU, and AN7973 (2 × EC90 = 0.42 µM) or DMSO (control). All parasites are shown in green (Vicia villosa lectin staining) and newly synthesized DNA (incorporated EdU) is shown in cyan. Arrows indicate EdU positive parasites, which are seen only in the absence of AN7973. Representative images from 2 independent experiments are shown. Scale bars are 5 µm. d Potency of AN7973 against asexual growth (0–48 h (black line and circles)) and asexual-to-sexual conversion (48–72 h (cyan line and squares)) of C. parvum (Bunch Grass Farms Iowa isolate). Data are the means and SD for each concentration with data combined from 2 independent experiments (n = 4 per experiment)
