Fig. 4
Gene and protein levels of senescence markers are increased in hypertrophic obesity. a–e Correlation of senescence markers and cell size in fresh SAT biopsies from lean, obese and obese T2D, GLB1 (P < 0.001), PAI1 (P < 0.001), TP53 (P < 0.003), CDKN2A/P16 INK4 (P < 0.001), and TGFB1 (P < 0.01), n = 28 biological independent samples. Comparisons were made with Spearman’s correlation coefficient. f Immunoblots of p53 and TNFα in SAT biopsies. g ZNF521 mRNA was reduced in cells with good differentiation measured as area with lipid droplets. Individuals from h. h Immunoblot from six individuals with different degree of differentiation. SVF cell were differentiated 14 days. a–e mRNA expression was first normalized to 18 S and then normalized to expression in undifferentiated sample (=1)
