Fig. 5
Cell senescence induces proinflammatory signals and reduces lipid accumulation. a Double staining of differentiated SVF cells with ORO and β-galactosidase. Result from two individuals with different degree of differentiation. Scale bar 200 µm. b TNFA vs. IL6 in whole adipose tissue, P < 0.001, n = 28 biological independent samples. Comparisons were made with Spearman’s correlation coefficient. mRNA expression was first normalized to 18 S and then normalized to expression in undifferentiated sample (=1). c–f Nutlin3a, inhibitor of p53 degradation, was added to the differentiation cocktail at time 0 h and was present throughout differentiation. Expression of adipogenic and senescence markers. Differentiation for 3 days (c–e) and 12 days (f). Results from five independent experiments, means ± SEM. f Images from differentiation day 10 of the cells in figure (e). Scale bar 100 µm. c–f Comparisons were made with Spearman’s correlation coefficient. *P < 0.05, **P < 0.01, and ***P < 0.001
