Fig. 3

Tonotopic connectivity differs between the left and right ACx. a Horizontal slices capture the anterior–posterior (A↔P) representation of tonotopy in the left and right ACx (top); white box scale 1 mm × 1 mm. DIC image of a horizontal slice during an experiment depicting the anatomical landmarks used to align consistently the stimulation grid (bottom). b, c Left, middle, and right panels are representative single-cell maps of six L3 neurons mapped at anterior, middle, and posterior locations of the left and right ACx, respectively. Each cell is shown with its native position along the tonotopic axis (arrows); scale bar 0.3 mm. d, e 2D population maps where each row is the average L6 input to L3 cells mapped within 50 μm from one another and ordered by tonotopic position in the left ACx (n = 38) and the right ACx (n = 40), respectively. The x axis is the position of the cells in the LSPS map grid (cells are always centered on their input maps). The tonotopic position of each row is the average distance of the cells binned to the tip of the hippocampus. Dashed white line represents the soma position and gray lines connect pixels of maximum input strength. Line plots in d and e are the columnar and row averages of synaptic input calculated from the 2D population maps. Asterisk indicates that synaptic input arising from the anterior and posterior areas of the 2D population map were significantly different (p = 1.4e-3, n = 38, Wilcoxon rank-sum). X scale bar 0.3 mm, y scale bar 0.33 mm. All data are presented as mean ± SEM